Shown below are examples of the specific stem cell counts determined for commonly studied tissue stem cell preparations.
In addition to determining the tissue stem cell fraction of any starting tissue cell preparation, the TORTOISE TestTM software has many other unique capabilities. It also provides for the first time the ability to quantify kinetic properties of tissue stem cells selectively without confounding with their ever present and more abundant progeny, committed progenitor cells. The figure below shows how, in addition to detecting changes in tissue stem cell number with cell culture passage, the TORTOISE TestTM software can delineate between the kinetics of tissue stem cells, transiently amplifying cells, and arrested differentiated cells in the same cell culture. The software also provides specific outputs for tissue stem cell viability and self-renewal kinetics. These analyses are powerful new tools for investigating tissue stem cell function in response to agents like drug candidates and in relationship to specific cellular mechanisms of interest.