In the past, it has been impossible to count tissue stem cells like bone marrow blood stem cells and tissue mesenchymal stem cells. The counting problem persisted because no biomarkers could be found that identify tissue stem cells specifically. All available biomarkers also count other tissue cell types that greatly outnumber tissue stem cells in all their preparations. Whether in research, for drug evaluations, in biomanufacturing processes, or in treatments, tissue stem cells are a small fraction of the total cells. So, even with sensitive methods like flow cytometry, because the available biomarkers are not specific, tissue stem cell counting has been impossible.
Asymmetrex’s Tortoise TestTM and Rabbit CountTM technologies solve this problem by using kinetic stem cell counting. This computational method does not require biomarkers and does not require that the stem cells be altered in any way. Kinetic counting is based on measuring the rate at which other cells are produced by the special division of tissue stem cells. It counts tissue stem cells in their natural state.